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microarray hybridization chamber kit  (Agilent technologies)


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    Agilent technologies microarray hybridization chamber kit
    Microarray Hybridization Chamber Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hybridization+chamber+kit/pmc10127108-156-15-19
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Labeling:

    Article Title: Exosomes Secreted by Toxoplasma gondii -Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
    Article Snippet: .. Labeled samples were subsequently hybridized onto a microarray slide using a hybridization chamber kit (Agilent Technologies, Santa Clara, California, USA) and hybridization gasket slide kit (Agilent Technologies). ..

    Article Title: miR-4463 regulates aromatase expression and activity for 17β-estradiol synthesis in response to follicle-stimulating hormone
    Article Snippet: .. The labeled samples were subsequently hybridized by loading onto a microarray slide using a Hybridization Chamber Kit (part #G2534A; Agilent Technologies, Santa Clara, CA, USA) and a Hybridization Gasket Slide Kit (part #G2534-60003; Agilent Technologies). ..

    Article Title: Exosomal miRNA-21 from Toxoplasma gondii -infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes
    Article Snippet: .. Labeled samples were subsequently hybridized by loading onto a microarray slide using Hybridization Chamber Kit (Agilent Technologies, Santa Clara, CA, USA) and Hybridization Gasket Slide Kit (Agilent Technologies). ..

    Microarray:

    Article Title: Exosomes Secreted by Toxoplasma gondii -Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
    Article Snippet: .. Labeled samples were subsequently hybridized onto a microarray slide using a hybridization chamber kit (Agilent Technologies, Santa Clara, California, USA) and hybridization gasket slide kit (Agilent Technologies). ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: miR-4463 regulates aromatase expression and activity for 17β-estradiol synthesis in response to follicle-stimulating hormone
    Article Snippet: .. The labeled samples were subsequently hybridized by loading onto a microarray slide using a Hybridization Chamber Kit (part #G2534A; Agilent Technologies, Santa Clara, CA, USA) and a Hybridization Gasket Slide Kit (part #G2534-60003; Agilent Technologies). ..

    Article Title: Exosomal miRNA-21 from Toxoplasma gondii -infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes
    Article Snippet: .. Labeled samples were subsequently hybridized by loading onto a microarray slide using Hybridization Chamber Kit (Agilent Technologies, Santa Clara, CA, USA) and Hybridization Gasket Slide Kit (Agilent Technologies). ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) .. 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) ..

    Hybridization:

    Article Title: Exosomes Secreted by Toxoplasma gondii -Infected L6 Cells: Their Effects on Host Cell Proliferation and Cell Cycle Changes
    Article Snippet: .. Labeled samples were subsequently hybridized onto a microarray slide using a hybridization chamber kit (Agilent Technologies, Santa Clara, California, USA) and hybridization gasket slide kit (Agilent Technologies). ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: miR-4463 regulates aromatase expression and activity for 17β-estradiol synthesis in response to follicle-stimulating hormone
    Article Snippet: .. The labeled samples were subsequently hybridized by loading onto a microarray slide using a Hybridization Chamber Kit (part #G2534A; Agilent Technologies, Santa Clara, CA, USA) and a Hybridization Gasket Slide Kit (part #G2534-60003; Agilent Technologies). ..

    Article Title: Exosomal miRNA-21 from Toxoplasma gondii -infected microglial cells induces the growth of U87 glioma cells by inhibiting tumor suppressor genes
    Article Snippet: .. Labeled samples were subsequently hybridized by loading onto a microarray slide using Hybridization Chamber Kit (Agilent Technologies, Santa Clara, CA, USA) and Hybridization Gasket Slide Kit (Agilent Technologies). ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) .. 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) ..

    Blocking Assay:

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) .. 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) ..

    Spectrophotometry:

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) .. 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) ..

    Extraction:

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) .. 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) ..

    Software:

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) .. 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) ..

    Control:

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) .. 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) ..

    Cell Culture:

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • • Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..

    Article Title: Array painting: a protocol for the rapid analysis of aberrant chromosomes using DNA microarrays
    Article Snippet: .. Agilent aCGH Wash Buffer 1 (Agilent, cat. no. 5188-5221) Agilent aCGH Wash Buffer 2 (Agilent, cat. no. 5188-5222) EQUIPMENT 0.22-μm syringe filter (Nalgene, cat. no. 190-2520) 20-μm mesh filter (Celltrics, Partec, cat. no. 04-004-2325) MoFlo High Performance Cell Sorter equipped with two water-cooled lasers (Coherent, Innova 300 series) 1.5-ml microfuge tubes Benchtop microfuge Heat block Thermal cycler Microcon YM-30, 30 kDa, sample reservoirs and tubes (Millipore, cat. no. 42422) NanoDrop 2000 Spectrophotometer (Thermo Scientific) Hybridization Gasket Slide Kit (5) for 1 microarray per slide format (Agilent, cat. no. G2534-60003) Hybridization Chamber Kit (Agilent, cat. no. G2534A) Blunt forceps (supplied with hybridization chamber) Human Genome CGH Microarray Kit 244A, consists of 5 microarrays (Agilent, cat. no. G4411B) DNA Microarray Hybridization Oven (Agilent, cat. no. G2545A) set to 65 °C, 20 rpm Hybridization Oven Rotator Rack (Agilent, cat. no. G2530-60029) Three glass troughs One slide rack Microarray Scanner (we used Agilent G2565CA but G2565BA can be used, Agilent) Slide holders supplied with scanner Feature Extraction 9.5 or 10.5 software and Agilent Scan Control software—both supplied with scanner DNA Analytics 4.0 (Agilent, cat. no. G4172AA) Freely available ‘R’ software Summit v3.1 (analysis software from Dako/Beckman Coulter) REAGENT SETUP Cell culture medium Supplement 500 ml of RMPI 1640 or DMEM with 15% (vol/vol) fetal calf serum and antibiotics. ..



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell

    Article Title: Single-cell RNA-seq reveals AML hierarchies relevant to disease progression and immunity

    doi: 10.1016/j.cell.2019.01.031

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Hybridization Chamber Kit - SureHyb enabled , Agilent , Cat# G2534A.

    Techniques: Recombinant, Hybridization, Luciferase, Transfection, Ligation, Sequencing, Mutagenesis, Clone Assay, Software